Application Information

This drug has been submitted to the FDA under the reference 009561/001.

Names and composition

"HYPAQUE" is the commercial name of a drug composed of DIATRIZOATE SODIUM.

Forms

ApplId/ProductId Drug name Active ingredient Form Strenght
009561/001 HYPAQUE DIATRIZOATE SODIUM INJECTABLE/INJECTION 50%
009561/003 HYPAQUE DIATRIZOATE SODIUM INJECTABLE/INJECTION 25%
011386/001 HYPAQUE DIATRIZOATE SODIUM FOR SOLUTION/ORAL, RECTAL 100%
011386/003 HYPAQUE DIATRIZOATE SODIUM SOLUTION/ORAL, RECTAL 40%
016403/001 HYPAQUE DIATRIZOATE MEGLUMINE INJECTABLE/INJECTION 60%
016403/002 HYPAQUE DIATRIZOATE MEGLUMINE INJECTABLE/INJECTION 30%

Similar Active Ingredient

ApplId/ProductId Drug name Active ingredient Form Strenght
009561/001 HYPAQUE DIATRIZOATE SODIUM INJECTABLE/INJECTION 50%
009561/002 HYPAQUE SODIUM 20% DIATRIZOATE SODIUM SOLUTION/URETERAL 20%
009561/003 HYPAQUE DIATRIZOATE SODIUM INJECTABLE/INJECTION 25%
011386/001 HYPAQUE DIATRIZOATE SODIUM FOR SOLUTION/ORAL, RECTAL 100%
011386/003 HYPAQUE DIATRIZOATE SODIUM SOLUTION/ORAL, RECTAL 40%
087075/001 MD-50 DIATRIZOATE SODIUM INJECTABLE/INJECTION 50%
087725/001 UROVIST SODIUM 300 DIATRIZOATE SODIUM INJECTABLE/INJECTION 50%

Ask a doctor

A licensed doctor will try to answer your question for free as quickly as possible. Free of charge during the beta period.

Answered questions

What is hypaque enema?
Asked by Marci Pradhan 1 year ago.

Hypaque Sodium (Diatrizoate Sodium, USP) is sodium 3,5-diacetamido-2, 4, 6-triiodobenzoate (C11H8I3N2NaO4) and contains 59.87 percent iodine. It is available as a powder. The powder (for preparing radiopaque solutions) provides about 600 mg organically bound iodine per gram of powder and contains caramel as a coloring agent. When administered orally or given as an enema, the medium produces excellent opacification and delineation of the upper and lower gastrointestinal tract; however, because of dilution, contrast in the small bowel may be unsatisfactory. Hypaque solutions are particularly valuable when a more viscous agent, such as barium sulfate which is not water soluble, is unsuitable or potentially harmful. Answered by Masako Jiggetts 1 year ago.

Hypaque Enema Answered by Melody Kellie 1 year ago.

I just realized your pun. Good one. I love puns. God is an it; it has no form that we could consider a body. Therefore, God needs no enemas. But you may. It really cleans you out, bro. As a physician, I suggest a thorough look into the Bible for weirdness. There is plenty of it in there. I'm not going to tell you the good/bad parts, but trust me every perversity that humans are capable of is in there. Go find it. Hint: Old Testament. Answered by Dominque Cushner 1 year ago.

This Site Might Help You. RE: What is hypaque enema? Answered by Delena Shamapande 1 year ago.


What is the density of ficoll hypaque?
ficoll hypaque used for density centrifugation to separate lymphocytes.n also density of red blood cells? Asked by Laquanda Colberg 1 year ago.

well, its tough to figure out what you need. Ficoll-Sodium Metrizoate is the chemical used... but also some of these use Percoll (an aqueous colloidal solution of polyvinylpyrrolidone-coated silica). So just search these 2 chemicals on yahoo for their densities. If you are lookin for how to calibrate the test: get some Percoll, equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density greater than 1.0 g/ml. which includes most types of lymph and parathyroid types... not sure about Lympho and Erythrocytes tho. Sorry! Answered by Sirena Quaid 1 year ago.


Can peripheral blood mononuclear cells (PBMC) be called a primary cell line?
Asked by Tressa Landquist 1 year ago.

Yes. If you isolate them (e.g. ficoll-hypaque or dish adhesion method) and use them, directly and living, in an experiment without inducing them to proliferate, that is a primary cell culture technique. Answered by Arcelia Hample 1 year ago.


What is the morphology of primary peritoneal murine macrophages immediately after culturing and first wash?
Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were... Asked by Lacie Dornon 1 year ago.

Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were adherent however most of them looked circular and very few were spindle shaped. Please anyone can help me to know what should be exact morphology of these cells after 16 hours and after how long I can expect well defined macrophages??? Answered by Pei Trousdale 1 year ago.

Isha - The two most convenient sources of primary murine macrophages are the bone marrow and the peritoneal cavity. Resident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an activated macrophage population can be achieved by using Bacillus-Calmette-Guerin as the inflammatory stimulus. Resident bone marrow macrophages can be isolated following enzymatic separation of cells from bone marrow plugs and enrichment on 30% fetal calf serum containing medium or Ficoll-Hypaque gradients. Bone marrow-derived macrophages can be produced by differentiating nonadherent macrophage precursors with medium containing macrophage colony-stimulating factor. Answered by Larraine Masoud 1 year ago.


What is hypaque enema?
Asked by Freida Fetzer 1 year ago.

Hypaque Sodium (Diatrizoate Sodium, USP) is sodium 3,5-diacetamido-2, 4, 6-triiodobenzoate (C11H8I3N2NaO4) and contains 59.87 percent iodine. It is available as a powder. The powder (for preparing radiopaque solutions) provides about 600 mg organically bound iodine per gram of powder and contains caramel as a coloring agent. When administered orally or given as an enema, the medium produces excellent opacification and delineation of the upper and lower gastrointestinal tract; however, because of dilution, contrast in the small bowel may be unsatisfactory. Hypaque solutions are particularly valuable when a more viscous agent, such as barium sulfate which is not water soluble, is unsuitable or potentially harmful. Answered by Setsuko Croyle 1 year ago.

Hypaque Enema Answered by Shantelle Hanline 1 year ago.

I just realized your pun. Good one. I love puns. God is an it; it has no form that we could consider a body. Therefore, God needs no enemas. But you may. It really cleans you out, bro. As a physician, I suggest a thorough look into the Bible for weirdness. There is plenty of it in there. I'm not going to tell you the good/bad parts, but trust me every perversity that humans are capable of is in there. Go find it. Hint: Old Testament. Answered by Reinaldo Evett 1 year ago.

This Site Might Help You. RE: What is hypaque enema? Answered by Lynell Stoecker 1 year ago.


What is the density of ficoll hypaque?
ficoll hypaque used for density centrifugation to separate lymphocytes.n also density of red blood cells? Asked by Rochelle Litchford 1 year ago.

well, its tough to figure out what you need. Ficoll-Sodium Metrizoate is the chemical used... but also some of these use Percoll (an aqueous colloidal solution of polyvinylpyrrolidone-coated silica). So just search these 2 chemicals on yahoo for their densities. If you are lookin for how to calibrate the test: get some Percoll, equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density greater than 1.0 g/ml. which includes most types of lymph and parathyroid types... not sure about Lympho and Erythrocytes tho. Sorry! Answered by Roma Rittle 1 year ago.


Can peripheral blood mononuclear cells (PBMC) be called a primary cell line?
Asked by Mathilde Menk 1 year ago.

Yes. If you isolate them (e.g. ficoll-hypaque or dish adhesion method) and use them, directly and living, in an experiment without inducing them to proliferate, that is a primary cell culture technique. Answered by Raymond Shaffen 1 year ago.


What is the morphology of primary peritoneal murine macrophages immediately after culturing and first wash?
Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were... Asked by Linda Siverly 1 year ago.

Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were adherent however most of them looked circular and very few were spindle shaped. Please anyone can help me to know what should be exact morphology of these cells after 16 hours and after how long I can expect well defined macrophages??? Answered by Lonnie Chaffin 1 year ago.

Isha - The two most convenient sources of primary murine macrophages are the bone marrow and the peritoneal cavity. Resident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an activated macrophage population can be achieved by using Bacillus-Calmette-Guerin as the inflammatory stimulus. Resident bone marrow macrophages can be isolated following enzymatic separation of cells from bone marrow plugs and enrichment on 30% fetal calf serum containing medium or Ficoll-Hypaque gradients. Bone marrow-derived macrophages can be produced by differentiating nonadherent macrophage precursors with medium containing macrophage colony-stimulating factor. Answered by Nickie Yielding 1 year ago.


What is hypaque enema?
Asked by Elfrieda Gulden 1 year ago.

Hypaque Sodium (Diatrizoate Sodium, USP) is sodium 3,5-diacetamido-2, 4, 6-triiodobenzoate (C11H8I3N2NaO4) and contains 59.87 percent iodine. It is available as a powder. The powder (for preparing radiopaque solutions) provides about 600 mg organically bound iodine per gram of powder and contains caramel as a coloring agent. When administered orally or given as an enema, the medium produces excellent opacification and delineation of the upper and lower gastrointestinal tract; however, because of dilution, contrast in the small bowel may be unsatisfactory. Hypaque solutions are particularly valuable when a more viscous agent, such as barium sulfate which is not water soluble, is unsuitable or potentially harmful. Answered by Asha Wayner 1 year ago.

Hypaque Enema Answered by Karry Sandavol 1 year ago.

I just realized your pun. Good one. I love puns. God is an it; it has no form that we could consider a body. Therefore, God needs no enemas. But you may. It really cleans you out, bro. As a physician, I suggest a thorough look into the Bible for weirdness. There is plenty of it in there. I'm not going to tell you the good/bad parts, but trust me every perversity that humans are capable of is in there. Go find it. Hint: Old Testament. Answered by Lavona Demien 1 year ago.

This Site Might Help You. RE: What is hypaque enema? Answered by Cicely Abdeldayen 1 year ago.


What is the density of ficoll hypaque?
ficoll hypaque used for density centrifugation to separate lymphocytes.n also density of red blood cells? Asked by Amy Grist 1 year ago.

well, its tough to figure out what you need. Ficoll-Sodium Metrizoate is the chemical used... but also some of these use Percoll (an aqueous colloidal solution of polyvinylpyrrolidone-coated silica). So just search these 2 chemicals on yahoo for their densities. If you are lookin for how to calibrate the test: get some Percoll, equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density greater than 1.0 g/ml. which includes most types of lymph and parathyroid types... not sure about Lympho and Erythrocytes tho. Sorry! Answered by Bettina Montoney 1 year ago.


Can peripheral blood mononuclear cells (PBMC) be called a primary cell line?
Asked by Idella Eppolito 1 year ago.

Yes. If you isolate them (e.g. ficoll-hypaque or dish adhesion method) and use them, directly and living, in an experiment without inducing them to proliferate, that is a primary cell culture technique. Answered by Maris Fuentes 1 year ago.


What is the morphology of primary peritoneal murine macrophages immediately after culturing and first wash?
Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were... Asked by Nevada Slinkard 1 year ago.

Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were adherent however most of them looked circular and very few were spindle shaped. Please anyone can help me to know what should be exact morphology of these cells after 16 hours and after how long I can expect well defined macrophages??? Answered by Selina Feddersen 1 year ago.

Isha - The two most convenient sources of primary murine macrophages are the bone marrow and the peritoneal cavity. Resident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an activated macrophage population can be achieved by using Bacillus-Calmette-Guerin as the inflammatory stimulus. Resident bone marrow macrophages can be isolated following enzymatic separation of cells from bone marrow plugs and enrichment on 30% fetal calf serum containing medium or Ficoll-Hypaque gradients. Bone marrow-derived macrophages can be produced by differentiating nonadherent macrophage precursors with medium containing macrophage colony-stimulating factor. Answered by Freda Grajales 1 year ago.


What is hypaque enema?
Asked by Ena Wearing 1 year ago.

Hypaque Sodium (Diatrizoate Sodium, USP) is sodium 3,5-diacetamido-2, 4, 6-triiodobenzoate (C11H8I3N2NaO4) and contains 59.87 percent iodine. It is available as a powder. The powder (for preparing radiopaque solutions) provides about 600 mg organically bound iodine per gram of powder and contains caramel as a coloring agent. When administered orally or given as an enema, the medium produces excellent opacification and delineation of the upper and lower gastrointestinal tract; however, because of dilution, contrast in the small bowel may be unsatisfactory. Hypaque solutions are particularly valuable when a more viscous agent, such as barium sulfate which is not water soluble, is unsuitable or potentially harmful. Answered by Carlene Hegge 1 year ago.

Hypaque Enema Answered by Silvana Mail 1 year ago.

I just realized your pun. Good one. I love puns. God is an it; it has no form that we could consider a body. Therefore, God needs no enemas. But you may. It really cleans you out, bro. As a physician, I suggest a thorough look into the Bible for weirdness. There is plenty of it in there. I'm not going to tell you the good/bad parts, but trust me every perversity that humans are capable of is in there. Go find it. Hint: Old Testament. Answered by Leo Singeltary 1 year ago.

This Site Might Help You. RE: What is hypaque enema? Answered by Kayleen Ueno 1 year ago.


What is the density of ficoll hypaque?
ficoll hypaque used for density centrifugation to separate lymphocytes.n also density of red blood cells? Asked by Filomena Yrigollen 1 year ago.

well, its tough to figure out what you need. Ficoll-Sodium Metrizoate is the chemical used... but also some of these use Percoll (an aqueous colloidal solution of polyvinylpyrrolidone-coated silica). So just search these 2 chemicals on yahoo for their densities. If you are lookin for how to calibrate the test: get some Percoll, equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density greater than 1.0 g/ml. which includes most types of lymph and parathyroid types... not sure about Lympho and Erythrocytes tho. Sorry! Answered by Carlita Mentel 1 year ago.


Can peripheral blood mononuclear cells (PBMC) be called a primary cell line?
Asked by Demetrius Chalender 1 year ago.

Yes. If you isolate them (e.g. ficoll-hypaque or dish adhesion method) and use them, directly and living, in an experiment without inducing them to proliferate, that is a primary cell culture technique. Answered by Porfirio Shur 1 year ago.


What is the morphology of primary peritoneal murine macrophages immediately after culturing and first wash?
Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were... Asked by Elli Stockhausen 1 year ago.

Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were adherent however most of them looked circular and very few were spindle shaped. Please anyone can help me to know what should be exact morphology of these cells after 16 hours and after how long I can expect well defined macrophages??? Answered by Mathew Stroker 1 year ago.

Isha - The two most convenient sources of primary murine macrophages are the bone marrow and the peritoneal cavity. Resident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an activated macrophage population can be achieved by using Bacillus-Calmette-Guerin as the inflammatory stimulus. Resident bone marrow macrophages can be isolated following enzymatic separation of cells from bone marrow plugs and enrichment on 30% fetal calf serum containing medium or Ficoll-Hypaque gradients. Bone marrow-derived macrophages can be produced by differentiating nonadherent macrophage precursors with medium containing macrophage colony-stimulating factor. Answered by Sherry Zeherquist 1 year ago.


What is hypaque enema?
Asked by Danial Deblanc 1 year ago.

Hypaque Sodium (Diatrizoate Sodium, USP) is sodium 3,5-diacetamido-2, 4, 6-triiodobenzoate (C11H8I3N2NaO4) and contains 59.87 percent iodine. It is available as a powder. The powder (for preparing radiopaque solutions) provides about 600 mg organically bound iodine per gram of powder and contains caramel as a coloring agent. When administered orally or given as an enema, the medium produces excellent opacification and delineation of the upper and lower gastrointestinal tract; however, because of dilution, contrast in the small bowel may be unsatisfactory. Hypaque solutions are particularly valuable when a more viscous agent, such as barium sulfate which is not water soluble, is unsuitable or potentially harmful. Answered by Nadia Reyez 1 year ago.

Hypaque Enema Answered by Julian Mailhot 1 year ago.

I just realized your pun. Good one. I love puns. God is an it; it has no form that we could consider a body. Therefore, God needs no enemas. But you may. It really cleans you out, bro. As a physician, I suggest a thorough look into the Bible for weirdness. There is plenty of it in there. I'm not going to tell you the good/bad parts, but trust me every perversity that humans are capable of is in there. Go find it. Hint: Old Testament. Answered by Luanna Bonaccorsi 1 year ago.

This Site Might Help You. RE: What is hypaque enema? Answered by Domenica Loffler 1 year ago.


What is the density of ficoll hypaque?
ficoll hypaque used for density centrifugation to separate lymphocytes.n also density of red blood cells? Asked by Drusilla Herout 1 year ago.

well, its tough to figure out what you need. Ficoll-Sodium Metrizoate is the chemical used... but also some of these use Percoll (an aqueous colloidal solution of polyvinylpyrrolidone-coated silica). So just search these 2 chemicals on yahoo for their densities. If you are lookin for how to calibrate the test: get some Percoll, equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density greater than 1.0 g/ml. which includes most types of lymph and parathyroid types... not sure about Lympho and Erythrocytes tho. Sorry! Answered by Nicholle Taft 1 year ago.


Can peripheral blood mononuclear cells (PBMC) be called a primary cell line?
Asked by Chaya Serini 1 year ago.

Yes. If you isolate them (e.g. ficoll-hypaque or dish adhesion method) and use them, directly and living, in an experiment without inducing them to proliferate, that is a primary cell culture technique. Answered by Elfrieda Dimitriou 1 year ago.


What is the morphology of primary peritoneal murine macrophages immediately after culturing and first wash?
Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were... Asked by Sharri Paquet 1 year ago.

Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were adherent however most of them looked circular and very few were spindle shaped. Please anyone can help me to know what should be exact morphology of these cells after 16 hours and after how long I can expect well defined macrophages??? Answered by Waneta Lanes 1 year ago.

Isha - The two most convenient sources of primary murine macrophages are the bone marrow and the peritoneal cavity. Resident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an activated macrophage population can be achieved by using Bacillus-Calmette-Guerin as the inflammatory stimulus. Resident bone marrow macrophages can be isolated following enzymatic separation of cells from bone marrow plugs and enrichment on 30% fetal calf serum containing medium or Ficoll-Hypaque gradients. Bone marrow-derived macrophages can be produced by differentiating nonadherent macrophage precursors with medium containing macrophage colony-stimulating factor. Answered by Laree Dashne 1 year ago.


What is hypaque enema?
Asked by Casie Piersall 1 year ago.

Hypaque Sodium (Diatrizoate Sodium, USP) is sodium 3,5-diacetamido-2, 4, 6-triiodobenzoate (C11H8I3N2NaO4) and contains 59.87 percent iodine. It is available as a powder. The powder (for preparing radiopaque solutions) provides about 600 mg organically bound iodine per gram of powder and contains caramel as a coloring agent. When administered orally or given as an enema, the medium produces excellent opacification and delineation of the upper and lower gastrointestinal tract; however, because of dilution, contrast in the small bowel may be unsatisfactory. Hypaque solutions are particularly valuable when a more viscous agent, such as barium sulfate which is not water soluble, is unsuitable or potentially harmful. Answered by Arnita Everline 1 year ago.

Hypaque Enema Answered by Domenica Lennan 1 year ago.

I just realized your pun. Good one. I love puns. God is an it; it has no form that we could consider a body. Therefore, God needs no enemas. But you may. It really cleans you out, bro. As a physician, I suggest a thorough look into the Bible for weirdness. There is plenty of it in there. I'm not going to tell you the good/bad parts, but trust me every perversity that humans are capable of is in there. Go find it. Hint: Old Testament. Answered by Adrien Quatrevingt 1 year ago.

This Site Might Help You. RE: What is hypaque enema? Answered by Mildred Juback 1 year ago.


What is the density of ficoll hypaque?
ficoll hypaque used for density centrifugation to separate lymphocytes.n also density of red blood cells? Asked by Ming Butz 1 year ago.

well, its tough to figure out what you need. Ficoll-Sodium Metrizoate is the chemical used... but also some of these use Percoll (an aqueous colloidal solution of polyvinylpyrrolidone-coated silica). So just search these 2 chemicals on yahoo for their densities. If you are lookin for how to calibrate the test: get some Percoll, equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density greater than 1.0 g/ml. which includes most types of lymph and parathyroid types... not sure about Lympho and Erythrocytes tho. Sorry! Answered by Gisele Willner 1 year ago.


Can peripheral blood mononuclear cells (PBMC) be called a primary cell line?
Asked by Genevie Narkier 1 year ago.

Yes. If you isolate them (e.g. ficoll-hypaque or dish adhesion method) and use them, directly and living, in an experiment without inducing them to proliferate, that is a primary cell culture technique. Answered by Rosalina Bounds 1 year ago.


What is the morphology of primary peritoneal murine macrophages immediately after culturing and first wash?
Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were... Asked by Huey Scheibelhut 1 year ago.

Recently I have isolated and cultured primary peritoneal murine macrophages maintaining it in DMEM supplied with FBS and antibiotics. After incubating the cells for 4 hours at 37 degrees washed the non-adherent cells with PBS and again incubated with complete DMEM. When observed the cells after 16 hours, they were adherent however most of them looked circular and very few were spindle shaped. Please anyone can help me to know what should be exact morphology of these cells after 16 hours and after how long I can expect well defined macrophages??? Answered by Marcelene Krampitz 1 year ago.

Isha - The two most convenient sources of primary murine macrophages are the bone marrow and the peritoneal cavity. Resident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an activated macrophage population can be achieved by using Bacillus-Calmette-Guerin as the inflammatory stimulus. Resident bone marrow macrophages can be isolated following enzymatic separation of cells from bone marrow plugs and enrichment on 30% fetal calf serum containing medium or Ficoll-Hypaque gradients. Bone marrow-derived macrophages can be produced by differentiating nonadherent macrophage precursors with medium containing macrophage colony-stimulating factor. Answered by Jonell Lybrand 1 year ago.


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