MSDS: Where can I find a list of the best TYPE of gloves to use depending on the chemicals that you will use?
The chemicals are UREA ELECTROPHRESIS, guanidine hydrochloride, and HCL.
Asked by Detra Teeters 1 year ago.
Nitrile is your best bet...electrophoresis is a process, not a chemical. Answered by Timmy Cosme 1 year ago.
Which of the following is a protein denatruring agent??
a. NaCl b. water c.urea d.ammonium sulphate e.guanidine hydrochloride
Asked by Sheba Veleta 1 year ago.
i believe its e.guanidine hydrochloride Answered by Viva Smaniotto 1 year ago.
guanidine hydrochloride or urea. Answered by Jeanetta Schulz 1 year ago.
What is the molecular weight for β-Galactosidase?
Asked by Lelia Luzier 1 year ago.
The molecular weight of Escherichia coli β-galactosidase was determined in 6m- and 8m-guanidine hydrochloride by meniscus-depletion sedimentation equilibrium, sedimentation velocity and viscosity. Sedimentation equilibrium revealed heterogeneity with the smallest component having a molecular weight of about 50000. At lower speeds, the apparent weight-average molecular weight is about 80000. By use of a calculation based on an empirical correlation for proteins that are random coils in 6m-guanidine hydrochloride, sedimentation velocity gave a molecular weight of 91000, and the intrinsic viscosity indicated a viscosity-average molecular weight of 84000. Heating in 6m-guanidine hydrochloride lowered the viscosity of β-galactosidase in a variable manner. So as you can see the molecular weight varies depending upon the conditions. Answered by Margareta Maupin 1 year ago.
CAS NUMBER required?
what is CAS number of following; 1. HEPTAKIS(2,6 DI-O-METHYL)-B-CYCLODEXTRIN 2. L-CYSTEINE HYDROCHLORIDE MONOHYDRATE (HS NUMBER 29309016000)3. GUANIDINE HYDROCHLORIDE (HS NUMBER 29252900900)4. 2- (cyclohexylamino) ethane sulphonic acid (HS number 29213099900)5. FETUIN...
Asked by Kathern Kreidler 1 year ago.
what is CAS number of following; 1. HEPTAKIS(2,6 DI-O-METHYL)-B-CYCLODEXTRIN 2. L-CYSTEINE HYDROCHLORIDE MONOHYDRATE (HS NUMBER 29309016000) 3. GUANIDINE HYDROCHLORIDE (HS NUMBER 29252900900) 4. 2- (cyclohexylamino) ethane sulphonic acid (HS number 29213099900) 5. FETUIN from fetal calf serum (HS number 30021091000) Answered by Allan Grantier 1 year ago.
When I searched the link asked me to pay I give you the link but not sure it will help you Answered by Rosy Manlove 1 year ago.
Are there any methods that can refold protein in PVDF membrane?
are there any methods or buffer that can refold protein in PVDF membrane?
Asked by Hollis Pendl 1 year ago.
I've had good luck by using a high concentration of chaotrope (urea, guanidine hydrochloride) to facilitate refolding, and then dialyze away the chaotrope. The big advantage here is the gradual reduction of the molar concentration of the chaotrope, whcih allows time for renaturation to occur... Hope this helps! Answered by Chelsey Pullins 1 year ago.
Using a 10x saline stock solution at 9% nacl make 200mls of 0.9 % nacl solution?
2) For Potassium chloride salt, make a working solution of 250 mls at 0.1 M.3) For KCl, make 500 mls of a 250 mM working solution.4) For KCl, make 100 mls of a 10X stock at 2M.a. Using the 10X stock, what would be the concentration in mM of a 1X working solution?b. Using the 10X stock, how many...
Asked by Angelo Gunlock 1 year ago.
2) For Potassium chloride salt, make a working solution of 250 mls at 0.1 M. 3) For KCl, make 500 mls of a 250 mM working solution. 4) For KCl, make 100 mls of a 10X stock at 2M. a. Using the 10X stock, what would be the concentration in mM of a 1X working solution? b. Using the 10X stock, how many grams of KCl would be in 50 mls of a 1X working solution? 5) Make 500 mls of a 250 mM solution of Glucose. 6) Make 100 mls of 100 mM Sucrose. 7) Make 5 mls of 50mM lysine. 8) Make 1 ml of 25 mM glutamic acid. 9) Make 500 mls of a 10X stock of Tris base at 200 mM. a. How many grams of Tris are in 200 mls of a 1X dilution? 10) Make 10mls of 100 µMole (micromole) of glutamic acid. 11) Normal saline is 0.9% w/v. What is the molarity in mM? 12) For guanidine hydrochloride, make 250 mls of a 3 M Stock solution Answered by Eusebia Bruen 1 year ago.
learn how to do dilutions C1 x V1 = C2 x V2 for the 1st question, you have 9% saline (10x solution) you want 0.2L of 0.9% saline this is a 1:10 dilution 20ml in a total of 200ml solution = 0.9% 9% x V1 = 0.9% x 0.2L V1 = 0.02L or 20ml Answered by Keshia Wieand 1 year ago.
Is there anything happening if Hydrochloric acid is mix with Nitroguanidine?
Asked by Candy Maddron 1 year ago.
You'd probably just be forming the guanidine hydrochloride salt... Answered by Caridad Yupe 1 year ago.
what is the basic general procedure for extracting DNA from cells?
Asked by Evangelina Hefti 1 year ago.
There are many variants, but usually the cells are first lysed using a soapy solution (that will break open the lipid membranes), proteins are broken down using proteinase K, and guanidine hydrochloride will denature all proteins. Then, the DNA (that should still be intact) is separated from the denatured cell debris by e.g. anion exchange columns. It should bind to the column, while all other components will flow through. The DNA is then later eluted from the column using water. Answered by Francisco Mayner 1 year ago.